A process that shows how big the fragments are. Samples of DNA are put in the wells of the gel substance
Analyze it
Since DNA is negatively charged, you put it on the negative side in the wells. It will be attracted to the positive side, the smaller the fragment the farther it will move towards the positive side.
Apply it
If there's a crime scene, you can match a suspects DNA to the DNA in a crime scene.
Synthesize
It reminds us of people swimming laps in a pool, smaller people are going to go farther then big ones.
Argue
It's a positive thing because you can match up DNA with other samples and tell how big each fragment of DNA is.
Has a radioactive single stranded DNA molecule for the probe. Combines gel electrophoresis and nucleic acid hybridization. Compares DNA samples with their particular size in a sequence and also the size of the restriction fragments that contain the sequence.
Analyze it
Each DNA sample mixed with same restriction enzyme, then each sample's restriction fragments are separated by electrophoresis. Next, the "blotting" occurs. With the gel being in a certain arrangement, capillary action pulls the alkaline solution upward through the gel, transferring the DNA to a sheet of nitrocellulose paper and denaturing it in the process. The single strands of DNA stuck to paper blot are positioned in bands corresponding to those on the gel.
Apply it
Can identify heterozygote carriers of mutant alleles associated with genetic diseases.
Synthesize
Southern Blotting reminds me of print making or paint transferring when you put paint on one paper and lay it on another and transfer the picture over
Argue
I argue for southern blotting because it is a way to combine gel electrophoresis and nucleic acid hybridization to identify mutant alleles and genetic diseases in heterozygote carriers.
Tiny amount of a large number of single-stranded DNA fragments representing different genes fixed to a glass slide in a tightly spaced array.
Analyze it
First in this process you isolate the mRNA, then make cDNA by reverse transcription, using flourescently labeled nucleotides. Next, apply cDNA mixture to a microarray. The cDNA hybridizes with any complementary DNA on the microarray. Rinse off excess cDNA, scan microarray for flourescence. Each flourescent spot represents a gene expressed in the tissue sample.
Apply it
You can find out what genes are expressed in a sample
Synthesize
The actual picture of a microarray reminds of a t.v. screen with a bunch of little particles. The expressed genes remind me of t.v. particles.
Argue
I argue for microarrays because you can find out how much a gene is expressed by the intensity of the fluorescence. Also, the resulting color at a spot reveals the relative levels of expression of a particular gene.
Gel Electrophoresis
http://www.wiziq.com/tutorial/22367-Gel-Electrophoresis-Animation
Southern Blotting
http://highered.mcgraw-hill.com/olcweb/cgi/pluginpop.cgi?it=swf::535::535::/sites/dl/free/0072437316/120078/bio_g.swf::Southern%20Blot
http://highered.mcgraw-hill.com/olcweb/cgi/pluginpop.cgi?it=swf::535::535::/sites/dl/free/0072437316/120078/micro50.swf::Microarray