Part OneGene Cloning with Bacterial Plasmids
Describe
A gene is held in a small, circular plasmid. The plasmid grows in the cells and when it divides an identical plasmid goes to another cell.
Analyze
A plasmid is isolated from a bacteria cell and foreign DNA is inserted into it. This is how it holds two kinds of DNA
Apply
If we are interested in a certain gene, we can get it and make more of it.
Synthesize
This reminds us of an assembly line because in an assembly line you don't mass produce things if they aren't perfect. But when you get the exact product that you are looking for you make tons of it!
Argue
We are for this because it gives scientists an advantage in their studies. It would be like giving them a supercharged bacteria to work with.
Media
ch7f1.jpg
Resources
7th edition ap bio book
http://www.sumanasinc.com/webcontent/animations/content/plasmidcloning.html

Nucleic Acid Hybridization
Describe
It is a short sequence with a radioactive probe that makes the gene of interest glow.
Analyze
The DNA of the gene is detected by its ability to base pair with a complementary sequence on another nucleic acid molecule. Complimentary molecule can be RNA or DNA and is called a nucleic acid probe.
Apply
This process identifies the gene of interest in colonies of cells.
Synthesize
This reminds us of a black light that makes florescent items show up. This is related because the probe makes the gene of interest show up.
Argue
We are for it because the collection of bacterial clones can be screened for different genes by using probes with different nucleotide sequences.
Media
external image NUCLEIC.gif
Resources
7th Edition AP Biology Book
http://www.sinauer.com/cooper5e/animation0410.html
Genomic Library
Describe
collection of bacteriophages or fragments of foreign DNA. Also, it is complete sets of plasmid clones.
Analyze
Complete set of plasmid clones, each carrying copies of particular segments from the initial genome
Apply
When a scientist needs a certain type of clone, they don't need to re-make it themselves.
Synthesize
This reminds us of the Internet in a way. The reasoning for this is because, for example, say you are doing a research paper. You need a fact. Instead of taking forever and go find a book or person who knows, you pull up google and have the answer in less that ten seconds.
Argue
We are for this because if a scientist is in the middle of an experiment and needs a certain bacteria with a certain trait he can just go find a library that has is instead of stopping and making his own.
Media
external image 10_23_genomic_library.jpg
Resources
7th edition ap bio book
http://www.learnerstv.com/animation/animation.php?ani=167&cat=biology
Polymerase Chain Reaction
Describe
specific target segment, within one or many DNA molecules. Can be copies many times.
Analyze
Process in which the DNA splits and a certain piece can be copied as many times as one desires.
Apply
Say a scientist needs a certain piece of DNA for testing, he can have an unlimited amount. If a scientist was given one strand of DNA and only had one chance, we wouldn't be nearly as advanced as we are now.
Synthesize
This reminds us of DNA replication because it's kind of the same process. When the DNA splits, primers come in and attach to the DNA strands but instead of just replicating, scientists alter the procedure and pretty much add their own nucleotides.
Argue
This is a great strategy in science. We are all for it because it gives scientists an unlimited of DNA to use.
Media
external image 300px-PCR.svg.png
Resources
7th Edition AP Biology Book
http://www.sumanasinc.com/webcontent/animations/content/pcr.html